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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00003329-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003329-D01, RRID:AB_10659344
- Product name
- HSPD1 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HSPD1 protein.
- Antigen sequence
MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGAD
ARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGS
PKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNT
NEEAGDGTTTATVLARSIAKEGFEKISKGANPVEI
RRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATIS
ANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDE
LEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVL
LSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDG
EALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKD
MAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIV
TKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEY
EKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDR
VTDALNATRAAVEEGIVLGGGCALLRCIPALDSLT
PANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLI
VEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVV
RTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGA
MGGMGGGMGGGMF- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPD1 expression in human liver.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPD1 expression in A-431.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HSPD1 expression in transfected 293T cell line (H00003329-T02) by HSPD1 MaxPab polyclonal antibody.Lane 1: HSPD1 transfected lysate(61.10 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to HSPD1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSPD1 transfected lysate using anti-HSPD1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSPD1 purified MaxPab mouse polyclonal antibody (B01P) (H00003329-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
