66041-1-Ig

antibody from Proteintech Group

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

66041-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#66041-1-Ig, RRID:AB_11041709

Product name

HSP60 antibody

Antibody type

Monoclonal

Description

HSP60 antibody (Cat. #66041-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

2F10E7

Vial size

20ul, 150ul

References

Submitted references

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American journal of physiology. Heart and circulatory physiology 2024 May 1;326(5):H1124-H1130

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Selenomethionine alleviates chronic heat stress-induced breast muscle injury and poor meat quality in broilers via relieving mitochondrial dysfunction and endoplasmic reticulum stress.

Jing J, Wang J, Xiang X, Yin S, Tang J, Wang L, Jia G, Liu G, Chen X, Tian G, Cai J, Kang B, Che L, Zhao H
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Human CCDC51 and yeast Mdm33 are functionally conserved mitochondrial inner membrane proteins that demarcate a subset of organelle fission events.

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bioRxiv : the preprint server for biology 2024 Mar 22;

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Kumar A, Gok MO, Nguyen KN, Connor OM, Reese ML, Wideman JG, Muñoz-Gómez SA, Friedman JR
Current biology : CB 2024 Jun 17;34(12):2606-2622.e9

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Tumor Necrosis Factor Receptor-2 Signals Clear-Cell Renal Carcinoma Proliferation via Phosphorylated 4E Binding Protein-1 and Mitochondrial Gene Translation.

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The American journal of pathology 2024 Jul;194(7):1374-1387

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Cell 2024 Jul 11;187(14):3619-3637.e27

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Overexpression of SESN1 improves mitochondrial damage and mitophagy, a potential therapeutic strategy for cognitive dysfunction after anaesthesia.

Sun L, Hong X, Wang D, Li Y
The European journal of neuroscience 2024 Jan;59(2):208-219

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Upregulation of PTPN1 aggravates endotoxemia-induced cardiac dysfunction through inhibiting mitophagy.

Song Q, Ma H, Zhu L, Qi Z, Lan Z, Liu K, Zhang H, Wang K, Wang N
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Visualization of Balbiani Body disassembly during human primordial follicle activation.

Amin R, Bukulmez O, Woodruff JB
microPublication biology 2023;2023

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Mifepristone inhibits hepatoma growth by enhancing the GR-HSP60-survivin interaction to facilitate survivin degradation.

Huang YH, Lin KH, Lai MW, Yeh CT
Journal of Cancer 2023;14(16):3066-3077

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UBQLN2 and HSP70 participate in Parkin-mediated mitophagy by facilitating outer mitochondrial membrane rupture.

Ma Q, Xin J, Peng Q, Li N, Sun S, Hou H, Ma G, Wang N, Zhang L, Tam KY, Dussmann H, Prehn JH, Wang H, Ying Z
EMBO reports 2023 Sep 6;24(9):e55859

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A DRP-like pseudoenzyme coordinates with MICOS to promote cristae architecture.

Kumar A, Gok MO, Nguyen KN, Reese ML, Wideman JG, Muñoz-Gómez SA, Friedman JR
bioRxiv : the preprint server for biology 2023 Oct 4;

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Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation.

Weng SW, Wu JC, Shen FC, Chang YH, Su YJ, Lian WS, Tai MH, Su CH, Chuang JH, Lin TK, Liou CW, Chu TH, Kao YH, Wang FS, Wang PW
Diabetologia 2023 May;66(5):913-930

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Deficiency of RAB39B Activates ER Stress-Induced Pro-apoptotic Pathway and Causes Mitochondrial Dysfunction and Oxidative Stress in Dopaminergic Neurons by Impairing Autophagy and Upregulating α-Synuclein.

Chiu CC, Weng YH, Yeh TH, Lu JC, Chen WS, Li AH, Chen YL, Wei KC, Wang HL
Molecular neurobiology 2023 May;60(5):2706-2728

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The First Transcriptomic Atlas of the Adult Lacrimal Gland Reveals Epithelial Complexity and Identifies Novel Progenitor Cells in Mice.

Delcroix V, Mauduit O, Lee HS, Ivanova A, Umazume T, Knox SM, de Paiva CS, Dartt DA, Makarenkova HP
Cells 2023 May 21;12(10)

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Substitution of PINK1 Gly411 modulates substrate receptivity and turnover.

Fiesel FC, Fričová D, Hayes CS, Coban MA, Hudec R, Bredenberg JM, Broadway BJ, Markham BN, Yan T, Boneski PK, Fiorino G, Watzlawik JO, Hou X, McCarty AM, Lewis-Tuffin LJ, Zhong J, Madden BJ, Ordureau A, An H, Puschmann A, Wszolek ZK, Ross OA, Harper JW, Caulfield TR, Springer W
Autophagy 2023 Jun;19(6):1711-1732

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Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress.

Jing J, He Y, Liu Y, Tang J, Wang L, Jia G, Liu G, Chen X, Tian G, Cai J, Che L, Kang B, Zhao H
Journal of animal science and biotechnology 2023 Jun 4;14(1):79

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The mitochondrial intermembrane space protein mitofissin drives mitochondrial fission required for mitophagy.

Fukuda T, Furukawa K, Maruyama T, Yamashita SI, Noshiro D, Song C, Ogasawara Y, Okuyama K, Alam JM, Hayatsu M, Saigusa T, Inoue K, Ikeda K, Takai A, Chen L, Lahiri V, Okada Y, Shibata S, Murata K, Klionsky DJ, Noda NN, Kanki T
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Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Zhong S, Chen W, Wang B, Gao C, Liu X, Song Y, Qi H, Liu H, Wu T, Wang R, Chen B
Redox biology 2023 Jul;63:102760

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Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage.

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Autophagy 2023 Jan;19(1):75-91

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DJ-1 inhibits ferroptosis in cerebral ischemia-reperfusion via ATF4/HSPA5 pathway.

Li Y, Chen T, Xue Y, Wang Y, Peng L, Wang C, Yu S
Neurochemistry international 2023 Dec;171:105628

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Organelle-dependent polyprotein designs enable stoichiometric expression of nitrogen fixation components targeted to mitochondria.

Yang J, Xiang N, Liu Y, Guo C, Li C, Li H, Cai S, Dixon R, Wang YP
Proceedings of the National Academy of Sciences of the United States of America 2023 Aug 22;120(34):e2305142120

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R17C Mutation in Photoreceptor Disc-Specific Protein, PRCD, Results in Additional Lipidation Altering Protein Stability and Subcellular Localization.

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International journal of molecular sciences 2022 Sep 16;23(18)

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Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C.

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mBio 2022 Oct 26;13(5):e0143422

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Mechanism of a new photosensitizer (TBZPy) in the treatment of high-risk human papillomavirus-related diseases.

Li Z, Xiao Z, Feng Y, Wang Q, Teng M
Photodiagnosis and photodynamic therapy 2022 Mar;37:102591

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Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.

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Autophagy 2022 Jun;18(6):1401-1415

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The interaction between E3 ubiquitin ligase Parkin and mitophagy receptor PHB2 links inner mitochondrial membrane ubiquitination to efficient mitophagy.

Sun S, Hou H, Ma G, Ma Q, Li N, Zhang L, Dong C, Cao M, Tam KY, Ying Z, Wang H
The Journal of biological chemistry 2022 Dec;298(12):102704

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HSP60 Regulates Lipid Metabolism in Human Ovarian Cancer.

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SARS-CoV-2 Causes Mitochondrial Dysfunction and Mitophagy Impairment.

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Frontiers in microbiology 2021;12:780768

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Mitophagy reporter mouse analysis reveals increased mitophagy activity in disuse-induced muscle atrophy.

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The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.

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Journal of cell science 2021 Jul 1;134(13)

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Exogenous Fatty Acids Modulate ER Lipid Composition and Metabolism in Breast Cancer Cells.

Rizzo AM, Colombo I, Montorfano G, Zava S, Corsetto PA
Cells 2021 Jan 16;10(1)

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ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage and cGAS/STING activation.

Hu M, Zhou M, Bao X, Pan D, Jiao M, Liu X, Li F, Li CY
The Journal of clinical investigation 2021 Feb 1;131(3)

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DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke.

Peng L, Zhou Y, Jiang N, Wang T, Zhu J, Chen Y, Li L, Zhang J, Yu S, Zhao Y
Journal of neuroinflammation 2020 Mar 9;17(1):81

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Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling.

Kalpage HA, Wan J, Morse PT, Lee I, Hüttemann M
Cells 2020 Aug 6;9(8)

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Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis.

Wang P, Geng J, Gao J, Zhao H, Li J, Shi Y, Yang B, Xiao C, Linghu Y, Sun X, Chen X, Hong L, Qin F, Li X, Yu JS, You H, Yuan Z, Zhou D, Johnson RL, Chen L
Nature communications 2019 Feb 14;10(1):755

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Potential urine proteomics biomarkers for primary nephrotic syndrome.

Choi YW, Kim YG, Song MY, Moon JY, Jeong KH, Lee TW, Ihm CG, Park KS, Lee SH
Clinical proteomics 2017;14:18

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BMI1, a new target of CK2α.

Banerjee Mustafi S, Chakraborty PK, Dwivedi SK, Ding K, Moxley KM, Mukherjee P, Bhattacharya R
Molecular cancer 2017 Mar 7;16(1):56

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The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Wang W, Wang L, Lu J, Siedlak SL, Fujioka H, Liang J, Jiang S, Ma X, Jiang Z, da Rocha EL, Sheng M, Choi H, Lerou PH, Li H, Wang X
Nature medicine 2016 Aug;22(8):869-78

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Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Geng J, Sun X, Wang P, Zhang S, Wang X, Wu H, Hong L, Xie C, Li X, Zhao H, Liu Q, Jiang M, Chen Q, Zhang J, Li Y, Song S, Wang HR, Zhou R, Johnson RL, Chien KY, Lin SC, Han J, Avruch J, Chen L, Zhou D
Nature immunology 2015 Nov;16(11):1142-52

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Tetrachlorobenzoquinone triggers the cleavage of Bid and promotes the cross-talk of extrinsic and intrinsic apoptotic signalings in pheochromocytoma (PC) 12 cells.

Hu L, Su C, Song X, Shi Q, Fu J, Xia X, Xu D, Song E, Song Y
Neurotoxicology 2015 Jul;49:149-57

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66041-1-Ig(HSPD1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using HSPD1 antibody 66041-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 66041-1-Ig(HSPD1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 66041-1-Ig(HSPD1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue