Ab169190

antibody from Aladdin Scientific

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab169190

Provider

Aladdin Scientific

Product name

Hsp60 Mouse mAb

Antibody type

Monoclonal

Description

ApplicationWB: 1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDaICC/IF: 1/200Flow: 1/800 - 1/200,000

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

EF02/2E10

Vial size

100_l,10_l,1ml,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Hsp60 Mouse mAb (Ab169190) - Western Blot All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 58 kDa Exposure time: 15.0 s 

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Hsp60 Mouse mAb (Ab169190) - Western Blot All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 4.0 s 

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Hsp60 Mouse mAb (Ab169190) - ICC/IF IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Hsp60 Mouse mAb (Ab169190) - Flow Cytometry Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).