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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-41400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HSP60 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Hela whole cell extract, HeLa cell lysate, antigen standard for HSPD1 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis ofHsp60 in the 1) absence and 2) presence of immunizing peptide in HeLa cell lysate using a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA1-41400) at 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HSP60 Polyclonal Antibody(Product # PA1-41400) and a 60 kDa band corresponding to HSP60 was observed across cell lines and tissue extracts tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), THP-1 (Lane 3), Jurkat (Lane 4), SW480 (Lane 5), A549 (Lane 6), NIH/3T3 (Lane 7), COS-7 (Lane 8) and tissue extracts of Mouse Brain (Lane 9) and Rat Brain (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
