AM12011PU-N

antibody from Acris Antibodies GmbH

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AM12011PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM12011PU-N, RRID:AB_10649161

Product name

anti HSP60

Antibody type

Monoclonal

Antigen

Human HSP60 produced through recombinant DNA methods in E. coli. Epitope: Amino acids 383-447

Host

Mouse

Isotype

IgG

Antibody clone number

LK-1

Vial size

50 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Gels were loaded with 1, 2.5, 5.0 and 7.5 µg/lane of HeLa and Raw204.4 cell lysates. 0.2 µg/ml primary antibody was used, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti Mouse IgG (H+L) secondary antibody at 1/20.000 dilution was used.