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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9974 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9974, RRID:AB_1711601
- Product name
- PPP1R13B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ASPP [corrected] and cancer.
ASPP--Apoptotic specific regulator of p53.
The ASPP family: deciding between life and death after DNA damage.
Trigiante G, Lu X
Nature reviews. Cancer 2006 Mar;6(3):217-26
Nature reviews. Cancer 2006 Mar;6(3):217-26
ASPP--Apoptotic specific regulator of p53.
Liu ZJ, Lu X, Zhong S
Biochimica et biophysica acta 2005 Sep 25;1756(1):77-80
Biochimica et biophysica acta 2005 Sep 25;1756(1):77-80
The ASPP family: deciding between life and death after DNA damage.
Slee EA, Lu X
Toxicology letters 2003 Apr 4;139(2-3):81-7
Toxicology letters 2003 Apr 4;139(2-3):81-7
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using PPP1R13B polyclonal antibody (Cat # PAB9974) to detect over-expressed PPP1R13B in MCF-7 cells (Lane 2, arrowhead).Lane 1 is a non-transfected control.Lane 3 is MCF-7 cells over-expressing TP53BP2.Cell extracts were electrophoresed and transferred to nitrocellulose.The membrane was probed with the primary antibody at a 1 : 1,000 dilution.The identity of the lower MW band at approximately 50 KDa is unknown.Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.
