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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX60278 - Provider product page
- Provider
- GeneTex
- Product name
- Fetuin A antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG/Fetuin A/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (GTX60278) at 1:200 in 4?C. Followed by conjugation to secondary antibody at 1:3000 90min in 37?C. Predicted band 31kD. Observed band size: 31kD.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of mouse liver (lane 1) and mouse intestine (lane 2) lysates using AHSG antibody at a dilution of 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Antigen: GTX60278, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;TMB staining; Read the data in MicroplateReader by 450
- Validation comment
- ELISA
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA analysis with 0..2ug/100ul antigen and serially diluted AHSG antibody (1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse embryo using AHSG antibody at a dilution of 1:200.
