R1589P

antibody from Acris Antibodies GmbH

Targeting: AHSG A2HS, FETUA, HSGA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1589P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1589P, RRID:AB_981252

Product name

anti Alpha-2-HS-glycoprotein

Antibody type

Polyclonal

Antigen

This purified antibody was prepared from Rabbit serum after repeated immunizations with a recombinant Human Fetuin (Alpha-2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

Reactivity

Human

Host

Goat

Vial size

0.5 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunoblot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by immunoblot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 µl of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1/10,000 and 1/20,000 showed strong reactivity by immunoblot. In this blot the antibody was used at a 1/10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1/5,000 dilution of IRDyeTM800 conjugated Donkey anti-Goat IgG for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.