GTX48479

antibody from GeneTex

Targeting: AHSG A2HS, FETUA, HSGA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

GTX48479

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48479, RRID:AB_11175841

Product name

Fetuin A antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Goat

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 µg of purified human fetuin. Lane 2 contains 5 µl of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Donkey anti-Goat IgG

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ?g of purified human fetuin. Lane 2 contains 5 ?l of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TBST. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Donkey anti-Goat IgG