Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
- Other assay [1]
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- Product number
- PA5-51594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fetuin A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GLIYRQPNCD DPETEEAALV AIDYINQNLP WGYKHTLNQI DEVKVWPQQP SGELFEIEID TLETTCHVLD PTPVARCSVR QLKEHAVEGD CDFQLLKLDG KFSVVYAKCD SSPDSAEDVR KVCQDCPLLA PLNDTRVVHA AKAALA Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 64%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Role of Wnt/β-Catenin Pathway Mediators in Aortic Valve Stenosis.
Khan K, Yu B, Kiwan C, Shalal Y, Filimon S, Cipro M, Shum-Tim D, Cecere R, Schwertani A
Frontiers in cell and developmental biology 2020;8:862
Frontiers in cell and developmental biology 2020;8:862
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fetuin A in control (vector only transfected HEK293T lysate) and AHSG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Fetuin A Polyclonal Antibody (Product # PA5-51594).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Fetuin an In human cell line Hep G2 using a Fetuin A Polyclonal Antibody (Product # PA5-51594) shows localization to the Golgi apparatus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human liver using Fetuin A Polyclonal Antibody (Product # PA5-51594).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human bone marrow, cerebral cortex, liver and lymph node using Fetuin A Polyclonal Antibody (Product # PA5-51594) (A) shows similar protein distribution across tissues to an independent Fetuin A Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human kidney using Fetuin A Polyclonal Antibody (Product # PA5-51594) shows moderate extra-cellular positivity and moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human stomach using Fetuin A Polyclonal Antibody (Product # PA5-51594) shows moderate positivity in extracellular matrix.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human skeletal muscle using Fetuin A Polyclonal Antibody (Product # PA5-51594) shows no cytoplasmic positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human cerebellum using Fetuin A Polyclonal Antibody (Product # PA5-51594) shows moderate cytoplasmic positivity in a subset of Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human bone marrow using Fetuin A Polyclonal Antibody (Product # PA5-51594).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human cerebral cortex using Fetuin A Polyclonal Antibody (Product # PA5-51594).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fetuin A in human lymph node using Fetuin A Polyclonal Antibody (Product # PA5-51594).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Fetuin A attenuates calcification in HAVICs and is associated with inactivation of Wnt signaling genes. (A) Representative images of endogenous Fetuin A in HAVICs localized to the golgi apparatus (G), endoplasmic reticulum (ER), and vesicles (V). Arrows point to regions of interest. Scale bar: 20 mum. (B) Representative Alizarin Red S staining images of HAVICs treated with different concentrations of Fetuin A. (C) Quantification of calcium deposition using acid extraction following Alizarin Red S stain of HAVIC relative to Crystal Violet stain. The data are represented as mean +- SEM. Significance level was set at P < 0.05. * P < 0.05, ** P < 0.01 compared to the specified group; n = 6 HAVIC lines were used for each group. One-way ANOVA followed by Tukey's post hoc multiple comparisons test was used for statistical analysis. (D) mRNA expression of osteogenic and Wnt signaling genes after treatment with Fetuin A. The data are represented as mean +- SEM. Significance level was set at P < 0.05. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to control groups; n = 6 HAVIC lines were used for each group. Students t -test was used for statistical analysis. (E) Representative Western blot of phosphorylated beta-catenin and beta-catenin protein expression in HAVICs treated with 10 muM Fetuin A in OSM. (F) Densitometry analysis of phosphorylated beta-catenin and beta-catenin protein expression in HAVICs treated with 10 muM Fetuin A in OSM. The data are represented as mean +-