PA5-51594

antibody from Invitrogen Antibodies

Targeting: AHSG A2HS, FETUA, HSGA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51594

Provider

Invitrogen Antibodies

Product name

Fetuin A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GLIYRQPNCD DPETEEAALV AIDYINQNLP WGYKHTLNQI DEVKVWPQQP SGELFEIEID TLETTCHVLD PTPVARCSVR QLKEHAVEGD CDFQLLKLDG KFSVVYAKCD SSPDSAEDVR KVCQDCPLLA PLNDTRVVHA AKAALA Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 64%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Role of Wnt/β-Catenin Pathway Mediators in Aortic Valve Stenosis.

Khan K, Yu B, Kiwan C, Shalal Y, Filimon S, Cipro M, Shum-Tim D, Cecere R, Schwertani A
Frontiers in cell and developmental biology 2020;8:862

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Fetuin an In human cell line Hep G2 using a Fetuin A Polyclonal Antibody (Product # PA5-51594) shows localization to the Golgi apparatus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Fetuin A attenuates calcification in HAVICs and is associated with inactivation of Wnt signaling genes. (A) Representative images of endogenous Fetuin A in HAVICs localized to the golgi apparatus (G), endoplasmic reticulum (ER), and vesicles (V). Arrows point to regions of interest. Scale bar: 20 mum. (B) Representative Alizarin Red S staining images of HAVICs treated with different concentrations of Fetuin A. (C) Quantification of calcium deposition using acid extraction following Alizarin Red S stain of HAVIC relative to Crystal Violet stain. The data are represented as mean +- SEM. Significance level was set at P < 0.05. * P < 0.05, ** P < 0.01 compared to the specified group; n = 6 HAVIC lines were used for each group. One-way ANOVA followed by Tukey's post hoc multiple comparisons test was used for statistical analysis. (D) mRNA expression of osteogenic and Wnt signaling genes after treatment with Fetuin A. The data are represented as mean +- SEM. Significance level was set at P < 0.05. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to control groups; n = 6 HAVIC lines were used for each group. Students t -test was used for statistical analysis. (E) Representative Western blot of phosphorylated beta-catenin and beta-catenin protein expression in HAVICs treated with 10 muM Fetuin A in OSM. (F) Densitometry analysis of phosphorylated beta-catenin and beta-catenin protein expression in HAVICs treated with 10 muM Fetuin A in OSM. The data are represented as mean +-