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- Antibody Data
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-02754 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-02754, RRID:AB_1520966
- Product name
- Goat Polyclonal Fetuin A/AHSG Antibody
- Antibody type
- Polyclonal
- Description
- IgG purified. Recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fetuin A/AHSG Antibody [NBP1-02754] - Immunoblot of Fetuin using anti-Human Fetuin (2-HS Glycoprotein) antibody at a 1:10,000 to 1:20,000 dilution. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ml of a 1:50 dilution of human serum. Data contributed by Dr. George Grunberger, Wayne State University.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fetuin A/AHSG Antibody [NBP1-02754] - Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ul of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10000 and 1:20000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Goat IgG (code # for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fetuin A/AHSG Antibody [NBP1-02754] - Staining of human liver tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
