LF-MA0147

antibody from Invitrogen Antibodies

Targeting: GC DBP, hDBP, VDBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LF-MA0147

Provider

Invitrogen Antibodies

Product name

Anti-GC Monoclonal Antibody (2B12)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

A suggested positive control for this product is human plasma.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2B12

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on 5 uL of Human Plasma (Lane 1). The blot was probed with Mouse Anti-GC Monoclonal Antibody (Product # LF-MA0147, 0.05 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 50 kDa band corresponding to GC was observed across the sample tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).