Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29067 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, BCL-1.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GC Polyclonal Antibody (Product # PA5-29067). Sample (30 µg of whole cell lysate). Lane A: BCL-1. 10% SDS PAGE. GC Polyclonal Antibody (Product # PA5-29067) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GC Polyclonal Antibody detects GC protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract.10% SDS-PAGE. GC Polyclonal Antibody (Product # PA5-29067) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GC was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: GC Polyclonal Antibody (Product # PA5-29067) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Vitamin D Binding Protein (Product # PA5-29067) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
