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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver. Predicted reactivity: Rabbit (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Disparities in Head and Neck Cancer: A Case for Chemoprevention with Vitamin D.
Ibrahimovic M, Franzmann E, Mondul AM, Weh KM, Howard C, Hu JJ, Goodwin WJ, Kresty LA
Nutrients 2020 Aug 29;12(9)
Nutrients 2020 Aug 29;12(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitamin D Binding Protein (Product # PA5-29082) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Modulation of vitamin D related proteins. Western blot results for CAL-27 and SCC-25 HNC cell lines following 2 uM vitamin D treatment. ND indicates no detection. Quantitation was normalized to GAPDH as a loading control and fold-change from vehicle reported.
