MA1-19013

antibody from Invitrogen Antibodies

Targeting: TF PRO1557, PRO2086

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19013

Provider

Invitrogen Antibodies

Product name

Transferrin Monoclonal Antibody (HTF-14)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody will not cross-react with sheep, canine, equine or bovine.

Antibody clone number

HTF-14

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human transferrin detected by mouse HTF-14 Monoclonal antibody (Product # MA1-19013). 1. hTransferrin; 5 µg/well (red. con.); 2. hTransferrin; 3 µg/well (red. con.); 3. hTransferrin; 1 µg/well (red. con.); M Low Range marker (Bio-Rad); 4. hTransferrin; 1 µg/well (non-red. con.); 5. hTransferrin; 3 µg/well (non-red. con.); 6. hTransferrin; 5 µg/well (non-red. con.).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Transferrin Monoclonal Antibody (HTF-14) (Product # MA1-19013) and a band at ~80 kDa corresponding to Transferrin was observed in Human plasma sample. Whole cell extracts of 2 µL of Human Plasma (Lane 1) was electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining of human placenta (paraffin sections) using anti-transferrin (HTF-14) Monoclonal antibody (Product # MA1-19013).