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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA1-19013 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transferrin Monoclonal Antibody (HTF-14)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. This antibody will not cross-react with sheep, canine, equine or bovine. A suggested positive control is placental tissue. Western Blot: non-reducing conditions; Functional Application: Blocking.
- Reactivity
- Human, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HTF-14
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Human transferrin detected by mouse HTF-14 Monoclonal antibody (Product # MA1-19013). 1. hTransferrin; 5 µg/well (red. con.); 2. hTransferrin; 3 µg/well (red. con.); 3. hTransferrin; 1 µg/well (red. con.); M Low Range marker (Bio-Rad); 4. hTransferrin; 1 µg/well (non-red. con.); 5. hTransferrin; 3 µg/well (non-red. con.); 6. hTransferrin; 5 µg/well (non-red. con.).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Transferrin Monoclonal Antibody (HTF-14) (Product # MA1-19013) and a band at ~80 kDa corresponding to Transferrin was observed in Human plasma sample. Whole cell extracts of 2 µL of Human Plasma (Lane 1) was electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human placenta (paraffin sections) using anti-transferrin (HTF-14) Monoclonal antibody (Product # MA1-19013).
