MA1-20106

antibody from Invitrogen Antibodies

Targeting: TF PRO1557, PRO2086

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA1-20106

Provider

Invitrogen Antibodies

Product name

Transferrin Monoclonal Antibody (12A6)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-20106 detects transferrin in human samples.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

12A6

Vial size

100 µg

Concentration

6.1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Transferrin Monoclonal Antibody (Product # MA1-20106) and a 80 kDa band corresponding to Transferrin was observed across the samples except HeLa conditioned media. 7 ul of Hep G2 conditioned media (Lane 1), 7 ul of HeLa conditioned media (Lane 2) and 2 ul of Human Plasma (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).