MIT0603

antibody from Invitrogen Antibodies

Targeting: TF PRO1557, PRO2086

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

MIT0603

Provider

Invitrogen Antibodies

Product name

Transferrin Monoclonal Antibody (F2H8G6)

Antibody type

Monoclonal

Antigen

Other

Description

MIT0603 has been successfully used in ELISA, immunofluorescence, immunoprecipitation, and radioimmune assay applications, and reacts with human samples. MIT0603 detects Transferrin which has a predicted molecular weight of approximately 67 kDa. MIT0603 was formerly sold as a Seradyn product.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

F2H8G6

Vial size

1 mg

Concentration

5 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100 µL of an transferrin chicken polyclonal antibody (Product # PA1-9525) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382) overnight at 4C. Wells were blocked with 100 µL of StartingBlock T20 (TBS) Blocking Buffer (Product # 28382) for 2 hours, and 100 µL of recombinant transferrin protein ranging from 1.6-1000 ng/mL were incubated for 1 hour at room temperature. The plate was washed with 1X TBST (Product # 28360), and 100 µL per well of an Transferrin mouse monoclonal antibody (Product # MIT0603) diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100 µL per well of a Streptavidin-HRP (Product # 21126) was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028), followed by Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Transferrin (green) in HepG2 cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Transferrin monoclonal antibody (Product # MIT0603), at a dilution of 1:100 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.