PA1-84854

antibody from Invitrogen Antibodies

Targeting: TF PRO1557, PRO2086

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA1-84854

Provider

Invitrogen Antibodies

Product name

Transferrin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

Goat anti Human Transferrin antibody recognizes human transferrin, an iron binding glycoprotein of approximately 79 kDa.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

1 mg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Transferrin Polyclonal Antibody (Product # PA1-84854) and a 80 kDa band corresponding to Transferrin was observed across the samples except in HeLa and HeLa conditioned media in Fig. a and was also observed in Hep G2 cells upon treatment with secretion blockers (FLI-06 and PTI) in Fig. b. The expression of Transferrin was not observed in K-562 as reported in literature. Whole cell extracts of Hep G2 (Lane 1), HeLa (Lane 2), 7 ul of Hep G2 conditioned media (Lane 3), 7 ul of HeLa conditioned media (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6) and 2ul of Human Plasma (Lane 7) in Fig. a; whole cell extracts (30ug lysate) of Hep G2 (Lane 1) Hep G2 treated with FLI-06 (10uM for 4hr) (Lane 2), Hep G2 treated with PTI (1X for 4hr) (Lane 3), K-562 (Lane 4), K-562 treated with FLI-06 (10uM for 4hr) (Lane 5) and K-562 treated with PTI (1X for 4hr) (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent with Novex® ECL Kit (Product # WP20005).