Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-84854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transferrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- Goat anti Human Transferrin antibody recognizes human transferrin, an iron binding glycoprotein of approximately 79 kDa.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Transferrin Polyclonal Antibody (Product # PA1-84854) and a 80 kDa band corresponding to Transferrin was observed across the samples except in HeLa and HeLa conditioned media in Fig. a and was also observed in Hep G2 cells upon treatment with secretion blockers (FLI-06 and PTI) in Fig. b. The expression of Transferrin was not observed in K-562 as reported in literature. Whole cell extracts of Hep G2 (Lane 1), HeLa (Lane 2), 7 ul of Hep G2 conditioned media (Lane 3), 7 ul of HeLa conditioned media (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6) and 2ul of Human Plasma (Lane 7) in Fig. a; whole cell extracts (30ug lysate) of Hep G2 (Lane 1) Hep G2 treated with FLI-06 (10uM for 4hr) (Lane 2), Hep G2 treated with PTI (1X for 4hr) (Lane 3), K-562 (Lane 4), K-562 treated with FLI-06 (10uM for 4hr) (Lane 5) and K-562 treated with PTI (1X for 4hr) (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent with Novex® ECL Kit (Product # WP20005).