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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- LS-C209284 - Provider product page
- Provider
- LSBio
- Product name
- TF / Transferrin Antibody (Lyophilized, Biotin) LS-C209284
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight alpha 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight alpha 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight alpha 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer (MB-070) 1hr at 4 degrees C. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4 degrees C. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
