Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C59393 - Provider product page
- Provider
- LSBio
- Product name
- TF / Transferrin Antibody LS-C59393
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at 4°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Human Transferrin Polyclonal Antibody-Multiplex Fluorescent Western blot. Rabbit anti-Transferrin (Anti-TRANSFERRIN (Human Serum) (RABBIT) Antibody lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842), and Mouse-anti-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.