Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- LS-C355262 - Provider product page
- Provider
- LSBio
- Product name
- TF / Transferrin Antibody (clone F2H8G6) LS-C355262
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- F2H8G6
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100ul of an transferrin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant transferrin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an transferrin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.