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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500848 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500848, RRID:AB_11126944
- Product name
- TF (Transferrin) mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)
- Antibody type
- Monoclonal
- Description
- TF (Transferrin) mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TF
- Isotype
- IgG
- Antibody clone number
- OTI5G2
- Vial size
- 100 µl
- Concentration
- 0.61 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (RC209184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF.(1:2000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody.(1:1000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TF mouse monoclonal antibody (TA500848) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF(RC209184).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209184 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TF antibody(TA500848), and then analyzed by flow cytometry.
- Validation comment
- FC
