LS-C745249

antibody from LSBio

Targeting: TF PRO1557, PRO2086

ELISA

Antibody data

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Validation data

Product number: LS-C745249 - Provider product page
Provider: LSBio
Product name: TF / Transferrin Antibody (Lyophilized) LS-C745249
Antibody type: Polyclonal
Description: Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Storage: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

TF protein structure - LS-C745249 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of rabbit Anti-Transferrin primary antibody. Lane 1: Human Transferrin. Lane 2: None. Primary antibody: Transferrin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 76-81 kDa. 76 kDa for Transferrin. Other band(s): Transferrin slice varients and isoforms.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Other band(s): none.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer (MB-070) 1hr at 4°C. Other band(s): none.