Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- CF500848 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TF Monoclonal Antibody (OTI5G2), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI5G2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (RC209184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TF. (1:200. Positive lysates LY400433 (100 µg) and LC400433 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 6 different cell lines by using anti-TF monoclonal antibody. (1:1000)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-TF mouse monoclonal antibody (TA500848) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF(RC209184).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-TF mouse monoclonal antibody (TA500848) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF(RC209184).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209184 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody (TA500848), and then analyzed by flow cytometry.
