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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab132212 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Transferrin Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Transferrin Antibody, Monoclonal (F3), could be used for WB, IHC and so on. Application: WB: 0.01-2µg/mL IHC: 5-30µg/mL Protein Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 361-683- Antibody clone number
- F3
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Transferrin Mouse mAb (Ab132212) - Western Blot All lanes: Transferrin Mouse mAb (Ab132212) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 78 kDa Exposure time: 3.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Transferrin Mouse mAb (Ab132212) - Western Blot All lanes: Transferrin Mouse mAb (Ab132212) at 1/2000 dilution Samples: Recombinant Human Transferrin Protein (rp155948) at 20 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 76 kDa Exposure time: 3.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Transferrin Mouse mAb (Ab132212) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 30 µg/mL Transferrin Mouse mAb (Ab132212) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
