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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transferrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, human plasma, mouse plasma. Predicted reactivity: Chimpanzee (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.12 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Complement Genetic Variants and FH Desialylation in S. pneumoniae-Haemolytic Uraemic Syndrome.
Gómez Delgado I, Corvillo F, Nozal P, Arjona E, Madrid Á, Melgosa M, Bravo J, Szilágyi Á, Csuka D, Veszeli N, Prohászka Z, Sánchez-Corral P
Frontiers in immunology 2021;12:641656
Frontiers in immunology 2021;12:641656
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transferrin Polyclonal Antibody detects Transferrin protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. Transferrin Polyclonal Antibody (Product # PA5-27306) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Desialylated FH/FHRs in samples from Hungarian SP-HUS patients. WB analyses of FH/FHRs in two plasma samples (sp1 and sp2) from four Hungarian patients. Time between disease onset and extraction date was as follows: HUN156 (10 days and 11 months); HUN816 (9 days and 2 months); HUN1869 (5 days and 2 months), this patient is homozygous for FHR-3 and FHR-1 deficiency; HUN2638 (23 days and 43 days). WB analysis of human Transferrin (Mw 77 kDa) in sp1 and sp2 samples from patients H837, HUN156, and HUN 1869 is shown at the bottom inset.
