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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- NB300-340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-340, RRID:AB_10002975
- Product name
- Chicken Polyclonal Transferrin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Transferrin
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transferrin Antibody [NB300-340] - Analysis of lysate expressing protein transferrin (NBL1-16827), probed with anti-transferrin antibody diluted at 1:1000. Lane 1: MW marker, Lane 2: Transferrin over-expression HEK293T cell lysate, Lane 3: Empty vector transfected control HEK293T cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transferrin Antibody [NB300-340] - Plasma Transferrin protein as antigen. IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-TF )
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Transferrin Antibody [NB300-340] - Recombinant protein as test antigen. Affinity-Purified anti-Transferrin IgY as primary antibody (0.1 ug/ml), and Rabbit anti-IgY-HRP as 2nd antibody. ( anti-TF )
