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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- MAB5087 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Apolipoprotein H/ApoH Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human Apolipoprotein H/ApoH in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Streptococcal M1 protein constructs a pathological host fibrinogen network.
Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
Nature 2011 Apr 7;472(7341):64-8
Nature 2011 Apr 7;472(7341):64-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 1 µg/mL of Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Apolipoprotein H/ApoH at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein H/ApoH by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 67 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
