AF6575

antibody from R&D Systems

Targeting: APOH B2G1, BG

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6575

Provider

R&D Systems

Product name

Mouse/Rat Apolipoprotein H/ApoH Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse and rat Apolipoprotein H/ApoH in Western blots and detects recombinant mouse Apolipoprotein H/ApoH in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Apolipoprotein H/ApoH is observed.

Reactivity

Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q01339


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Gram Negative Bacterial Inflammation Ameliorated by the Plasma Protein Beta 2-Glycoprotein I.

Zhou S, Chen G, Qi M, El-Assaad F, Wang Y, Dong S, Chen L, Yu D, Weaver JC, Beretov J, Krilis SA, Giannakopoulos B
Scientific reports 2016 Sep 27;6:33656

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of rat liver tissue and mouse serum. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse/Rat Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein H/ApoH at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.