Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47696 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated) using 10 µg/mL of Goat Anti-human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47696) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipoprotein H in human liver tissue. Samples were incubated in Apolipoprotein H polyclonal antibody (Product # PA5-47696) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipoprotein H in human liver tissue. Samples were incubated in Apolipoprotein H polyclonal antibody (Product # PA5-47696) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipoprotein H in 0.2 mg/mL lysates of human liver tissue. Samples were incubated in Apolipoprotein H polyclonal antibody (Product # PA5-47696) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 1:50. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Apolipoprotein H Polyclonal Antibody (Product # PA5-47696) and a 50 kDa band corresponding to Beta-2-glycoprotein 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X for 4 hours) (Lane 2), PC-3 (Lane 3), PC-3 treated with PTI (1X for 4 hours) (Lane 4), BeWo (Lane 5), BeWo treated with PTI (1X for 4 hours) (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Apolipoprotein H in perfusion fixed frozen sections of adult mouse pancreas. Samples were incubated in Apolipoprotein H polyclonal antibody (Product # PA5-47696) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets.