MAB5087

antibody from R&D Systems

Targeting: APOH B2G1, BG

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5087

Provider

R&D Systems

Product name

Human Apolipoprotein H/ApoH Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human Apolipoprotein H/ApoH in Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P02749


Isotype

IgG

Antibody clone number

517038

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Streptococcal M1 protein constructs a pathological host fibrinogen network.

Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
Nature 2011 Apr 7;472(7341):64-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 1 µg/mL of Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Apolipoprotein H/ApoH at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Apolipoprotein H/ApoH by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 67 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.