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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-65177 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOPBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KYEAAKKWNLP AVTIAWLLET ARTGKRADES HFLIENSTKE ERSLETEITN GINLNSDTAE HPGTRLQTHR KTVVTPLDMN RFQSKAFRAV VS Highest antigen sequence identity to the following orthologs - mouse 68%, rat 66%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TOPBP1 Polyclonal Antibody (Product # PA5-65177) and a 200 kDa band corresponding to TOPBP1 was observed in Mouse Heart, Mouse Skeletal Muscle and Rat Heart along with an uncharacterized band (*) at 160 kDa; and was absent in Mouse Kidney and Rat Kidney. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Kidney (Lane 3), Rat Heart (Lane 4) and Rat Kidney (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TOPBP1 in human cell line A-431 shows localization to nucleus and nuclear bodies. Samples were probed using a TOPBP1 Polyclonal Antibody (Product # PA5-65177).
