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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-96424 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOPBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KIITIEETHE ELKKQYIFQL SSLNPQERID YCHLIEKLGG LVIEKQCFDP TCTHIVVGHP LRNEKYLASV AAGKWVLHRS YLEACRTAGH FVQEEDYEWG SSSILDVLTG INVQQRRLAL AAMRWRKKIQ QRQESGIVEG AFSGWKVILH VDQSREAGFK RLLQSGGAKV LPGHSVPLFK EATHLFSDLN KLKPDDSGVN IAEAAAQNVY CLRTEYIADY LMQESPPHVE NYCLPEAISF IQNNKELGTG LSQKRKAPTE KNKIKRPRVH; Positive Samples: 293T; Cellular Location: Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TOPBP1 was performed in A549 cells using TOPBP1 Polyclonal Antibody (Product # PA5-96424).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOPBP1 in paraffin-embedded rat lung. Samples were incubated with TOPBP1 Polyclonal antibody (Product # PA5-96424) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOPBP1 in paraffin-embedded mouse intestine. Samples were incubated with TOPBP1 Polyclonal antibody (Product # PA5-96424) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOPBP1 in paraffin-embedded human colon carcinoma. Samples were incubated with TOPBP1 Polyclonal antibody (Product # PA5-96424) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOPBP1 in paraffin-embedded mouse intestine. Samples were incubated with TOPBP1 Polyclonal antibody (Product # PA5-96424) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
