PA5-96424

antibody from Invitrogen Antibodies

Targeting: TOPBP1 Dpb11, KIAA0259, TOP2BP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96424

Provider

Invitrogen Antibodies

Product name

TOPBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KIITIEETHE ELKKQYIFQL SSLNPQERID YCHLIEKLGG LVIEKQCFDP TCTHIVVGHP LRNEKYLASV AAGKWVLHRS YLEACRTAGH FVQEEDYEWG SSSILDVLTG INVQQRRLAL AAMRWRKKIQ QRQESGIVEG AFSGWKVILH VDQSREAGFK RLLQSGGAKV LPGHSVPLFK EATHLFSDLN KLKPDDSGVN IAEAAAQNVY CLRTEYIADY LMQESPPHVE NYCLPEAISF IQNNKELGTG LSQKRKAPTE KNKIKRPRVH; Positive Samples: 293T; Cellular Location: Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TOPBP1 in extracts of 293T cells using TOPBP1 Polyclonal Antibody (Product # PA5-96424) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TOPBP1 was performed in A549 cells using TOPBP1 Polyclonal Antibody (Product # PA5-96424).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TOPBP1 in paraffin-embedded mouse heart using TOPBP1 Polyclonal Antibody (Product # PA5-96424) at a dilution of 1:200.