Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 11486-2-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11486-2-AP, RRID:AB_2058396
- Product name
- APOL1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated APOL1 antibody (Cat. #11486-2-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Apolipoprotein L1 (APOL1) renal risk variant-mediated podocyte cytotoxicity depends on African haplotype and surface expression.
APOL1 Induces Pyroptosis of Fibroblasts Through NLRP3/Caspase-1/GSDMD Signaling Pathway in Ulcerative Colitis.
Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes.
HDL Isolated by Immunoaffinity, Ultracentrifugation, or Precipitation is Compositionally and Functionally Distinct.
Interplay of Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection.
Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif.
Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.
APOL1 risk variants affect podocyte lipid homeostasis and energy production in focal segmental glomerulosclerosis.
All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.
Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.
Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.
Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.
Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei.
MiR193a Modulation and Podocyte Phenotype.
Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.
Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.
The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.
A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.
Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.
BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.
Evolution of the primate trypanolytic factor APOL1.
Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins.
Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.
Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.
Gupta N, Waas B, Austin D, De Mazière AM, Kujala P, Stockwell AD, Li T, Yaspan BL, Klumperman J, Scales SJ
Scientific reports 2024 Feb 14;14(1):3765
Scientific reports 2024 Feb 14;14(1):3765
APOL1 Induces Pyroptosis of Fibroblasts Through NLRP3/Caspase-1/GSDMD Signaling Pathway in Ulcerative Colitis.
Zhu F, Li S, Gu Q, Xie N, Wu Y
Journal of inflammation research 2023;16:6385-6396
Journal of inflammation research 2023;16:6385-6396
Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes.
Vandorpe DH, Heneghan JF, Waitzman JS, McCarthy GM, Blasio A, Magraner JM, Donovan OG, Schaller LB, Shah SS, Subramanian B, Riella CV, Friedman DJ, Pollak MR, Alper SL
Pflugers Archiv : European journal of physiology 2023 Mar;475(3):323-341
Pflugers Archiv : European journal of physiology 2023 Mar;475(3):323-341
HDL Isolated by Immunoaffinity, Ultracentrifugation, or Precipitation is Compositionally and Functionally Distinct.
Holzer M, Ljubojevic-Holzer S, Souza Junior DR, Stadler JT, Rani A, Scharnagl H, Ronsein GE, Marsche G
Journal of lipid research 2022 Dec;63(12):100307
Journal of lipid research 2022 Dec;63(12):100307
Interplay of Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection.
Pant J, Samanovic M, Nelson MT, Keceli MK, Verdi J, Beverley SM, Raper J
PLoS pathogens 2021 Sep;17(9):e1008768
PLoS pathogens 2021 Sep;17(9):e1008768
Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif.
Ultsch M, Holliday MJ, Gerhardy S, Moran P, Scales SJ, Gupta N, Oltrabella F, Chiu C, Fairbrother W, Eigenbrot C, Kirchhofer D
Communications biology 2021 Jul 27;4(1):916
Communications biology 2021 Jul 27;4(1):916
Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.
Yu J, Lang Q, Zhong C, Wang S, Tian Y
Dose-response : a publication of International Hormesis Society 2021 Apr-Jun;19(2):15593258211023260
Dose-response : a publication of International Hormesis Society 2021 Apr-Jun;19(2):15593258211023260
APOL1 risk variants affect podocyte lipid homeostasis and energy production in focal segmental glomerulosclerosis.
Ge M, Molina J, Ducasa GM, Mallela SK, Varona Santos J, Mitrofanova A, Kim JJ, Liu X, Sloan A, Mendez AJ, Banerjee S, Liu S, Szeto HH, Shin MK, Hoek M, Kopp JB, Fontanesi F, Merscher S, Fornoni A
Human molecular genetics 2021 Apr 26;30(3-4):182-197
Human molecular genetics 2021 Apr 26;30(3-4):182-197
All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.
Verdi J, Schaub C, Thomson R, Raper J
Methods in molecular biology (Clifton, N.J.) 2020;2116:463-483
Methods in molecular biology (Clifton, N.J.) 2020;2116:463-483
Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.
Gupta N, Wang X, Wen X, Moran P, Paluch M, Hass PE, Heidersbach A, Haley B, Kirchhofer D, Brezski RJ, Peterson AS, Scales SJ
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2065-2082
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2065-2082
Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.
Scales SJ, Gupta N, De Mazière AM, Posthuma G, Chiu CP, Pierce AA, Hötzel K, Tao J, Foreman O, Koukos G, Oltrabella F, Klumperman J, Lin W, Peterson AS
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2044-2064
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2044-2064
Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.
Giovinazzo JA, Thomson RP, Khalizova N, Zager PJ, Malani N, Rodriguez-Boulan E, Raper J, Schreiner R
eLife 2020 May 19;9
eLife 2020 May 19;9
Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei.
Xu ZS, Li FJ, Hide G, Lun ZR, Lai DH
Parasites & vectors 2020 Apr 25;13(1):214
Parasites & vectors 2020 Apr 25;13(1):214
MiR193a Modulation and Podocyte Phenotype.
Jha A, Saha S, Ayasolla K, Vashistha H, Malhotra A, Skorecki K, Singhal PC
Cells 2020 Apr 17;9(4)
Cells 2020 Apr 17;9(4)
Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.
Aghajan M, Booten SL, Althage M, Hart CE, Ericsson A, Maxvall I, Ochaba J, Menschik-Lundin A, Hartleib J, Kuntz S, Gattis D, Ahlström C, Watt AT, Engelhardt JA, Monia BP, Magnone MC, Guo S
JCI insight 2019 Jun 20;4(12)
JCI insight 2019 Jun 20;4(12)
Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.
Yamane D, Feng H, Rivera-Serrano EE, Selitsky SR, Hirai-Yuki A, Das A, McKnight KL, Misumi I, Hensley L, Lovell W, González-López O, Suzuki R, Matsuda M, Nakanishi H, Ohto-Nakanishi T, Hishiki T, Wauthier E, Oikawa T, Morita K, Reid LM, Sethupathy P, Kohara M, Whitmire JK, Lemon SM
Nature microbiology 2019 Jul;4(7):1096-1104
Nature microbiology 2019 Jul;4(7):1096-1104
The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.
Zhang X, Hong XK, Li SJ, Lai DH, Hide G, Lun ZR, Wen YZ
Experimental parasitology 2018 Jan;184:115-120
Experimental parasitology 2018 Jan;184:115-120
A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.
Liu X, Zheng W, Wang W, Shen H, Liu L, Lou W, Wang X, Yang P
British journal of cancer 2017 Dec 5;117(12):1846-1854
British journal of cancer 2017 Dec 5;117(12):1846-1854
Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.
Kotb AM, Simon O, Blumenthal A, Vogelgesang S, Dombrowski F, Amann K, Zimmermann U, Endlich K, Endlich N
PloS one 2016;11(5):e0153768
PloS one 2016;11(5):e0153768
BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.
Heneghan JF, Vandorpe DH, Shmukler BE, Giovinazzo JA, Raper J, Friedman DJ, Pollak MR, Alper SL
American journal of physiology. Cell physiology 2015 Sep 1;309(5):C332-47
American journal of physiology. Cell physiology 2015 Sep 1;309(5):C332-47
Evolution of the primate trypanolytic factor APOL1.
Thomson R, Genovese G, Canon C, Kovacsics D, Higgins MK, Carrington M, Winkler CA, Kopp J, Rotimi C, Adeyemo A, Doumatey A, Ayodo G, Alper SL, Pollak MR, Friedman DJ, Raper J
Proceedings of the National Academy of Sciences of the United States of America 2014 May 20;111(20):E2130-9
Proceedings of the National Academy of Sciences of the United States of America 2014 May 20;111(20):E2130-9
Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins.
Sun HY, Chen SF, Lai MD, Chang TT, Chen TL, Li PY, Shieh DB, Young KC
Clinica chimica acta; international journal of clinical chemistry 2010 Mar;411(5-6):336-44
Clinica chimica acta; international journal of clinical chemistry 2010 Mar;411(5-6):336-44
Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.
Thomson R, Molina-Portela P, Mott H, Carrington M, Raper J
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 17;106(46):19509-14
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 17;106(46):19509-14
Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.
Molina-Portela MP, Samanovic M, Raper J
The Journal of experimental medicine 2008 Aug 4;205(8):1721-8
The Journal of experimental medicine 2008 Aug 4;205(8):1721-8
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human blood tissue were subjected to SDS PAGE followed by western blot with 11486-2-AP(APOL1 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using APOL1 antibody 11486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-APOL1 (IP:11486-2-AP, 3ug; Detection:11486-2-AP 1:500) with HepG2 cells lysate 6000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 11486-2-AP(APOL1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 11486-2-AP(APOL1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue