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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP7361a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7361a, RRID:AB_1967239
- Product name
- APOL1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- APOL1-BH3 Domain Antibody (Cat. #AP7361a) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the APOL1-BH3 Domain antibody detected the APOL1-BH3 Domain protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- APOL1 Antibody (Cat. #AP7361a) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOL1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- APOL1 Antibody (19169) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
