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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [6]
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- Product number
- LS-C375243 - Provider product page
- Provider
- LSBio
- Product name
- APOL1 / Apolipoprotein L Antibody (aa179-398) LS-C375243
- Antibody type
- Polyclonal
- Description
- Antigen Affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at -20°C. Long term: store at -80°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: Apolipoprotein L1 antibody at 10 ug/ml. Lane 1: A549 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: HepG2 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 44 kDa. Observed band size: 44 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: Apolipoprotein L1 antibody at 10µg/ml Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 44, 46, 43 kDa Observed band size: 44 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: APOL1 antibody at 1.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 46, 43 kDa Observed band size: 44 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
