Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-89033 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89033, RRID:AB_11052365
- Product name
- Rabbit Polyclonal Apolipoprotein L1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Apolipoprotein L1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Diabetes-related changes in the protein composition and the biomechanical properties of human retinal vascular basement membranes.
APOL1 null alleles from a rural village in India do not correlate with glomerulosclerosis.
APOL1 localization in normal kidney and nondiabetic kidney disease.
Halfter W, Moes S, Asgeirsson DO, Halfter K, Oertle P, Melo Herraiz E, Plodinec M, Jenoe P, Henrich PB
PloS one 2017;12(12):e0189857
PloS one 2017;12(12):e0189857
APOL1 null alleles from a rural village in India do not correlate with glomerulosclerosis.
Johnstone DB, Shegokar V, Nihalani D, Rathore YS, Mallik L, Ashish, Zare V, Ikizler HO, Powar R, Holzman LB
PloS one 2012;7(12):e51546
PloS one 2012;7(12):e51546
APOL1 localization in normal kidney and nondiabetic kidney disease.
Madhavan SM, O'Toole JF, Konieczkowski M, Ganesan S, Bruggeman LA, Sedor JR
Journal of the American Society of Nephrology : JASN 2011 Nov;22(11):2119-28
Journal of the American Society of Nephrology : JASN 2011 Nov;22(11):2119-28
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Simple Western lane view shows a specific band for APOL1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Electropherogram image(s) of corresponding Simple Western lane view. Apolipoprotein L1 antibody was used at 1:30 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of human colon shows positivity in plasma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of human skin shows positivity in plasma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.