HPA018885

antibody from Atlas Antibodies

Targeting: APOL1 APOL

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA018885

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA018885, RRID:AB_1844953

Product name

Anti-APOL1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human APOL1, Gene description: apolipoprotein L, 1, Alternative Gene Names: APOL, Validated applications: WB, IHC, Uniprot ID: O14791, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.3 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Apolipoprotein L1 (APOL1) renal risk variant-mediated podocyte cytotoxicity depends on African haplotype and surface expression

Gupta N, Waas B, Austin D, De Mazière A, Kujala P, Stockwell A, Li T, Yaspan B, Klumperman J, Scales S
Scientific Reports 2024;14(1)

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Apolipoproteins L1 and L3 control mitochondrial membrane dynamics

Lecordier L, Heo P, Graversen J, Hennig D, Skytthe M, Cornet d’Elzius A, Pincet F, Pérez-Morga D, Pays E
Cell Reports 2023;42(12):113528

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Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes

Vandorpe D, Heneghan J, Waitzman J, McCarthy G, Blasio A, Magraner J, Donovan O, Schaller L, Shah S, Subramanian B, Riella C, Friedman D, Pollak M, Alper S
Pflügers Archiv - European Journal of Physiology 2022;475(3):323-341

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DGAT2 Inhibition Potentiates Lipid Droplet Formation To Reduce Cytotoxicity in APOL1 Kidney Risk Variants

Chun J, Riella C, Chung H, Shah S, Wang M, Magraner J, Ribas G, Ribas H, Zhang J, Alper S, Friedman D, Pollak M
Journal of the American Society of Nephrology 2022;33(5):889-907

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Endoplasmic reticulum-translocation is essential for APOL1 cellular toxicity

Kruzel-Davila E, Bavli-Kertselli I, Ofir A, Cheatham A, Shemer R, Zaknoun E, Chornyy S, Tabachnikov O, Davis S, Khatua A, Skorecki K, Popik W
iScience 2022;25(1):103717

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Comparative Analysis of the APOL1 Variants in the Genetic Landscape of Renal Carcinoma Cells

Tzukerman M, Shamai Y, Abramovich I, Gottlieb E, Selig S, Skorecki K
Cancers 2022;14(3):733

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Evolution of Renal-Disease Factor APOL1 Results in Cis and Trans Orientations at the Endoplasmic Reticulum That Both Show Cytotoxic Effects

Müller D, Schmitz J, Fischer K, Granado D, Groh A, Krausel V, Lüttgenau S, Amelung T, Pavenstädt H, Weide T, Malik H
Molecular Biology and Evolution 2021;38(11):4962-4976

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APOL1 risk variants affect podocyte lipid homeostasis and energy production in focal segmental glomerulosclerosis

Ge M, Molina J, Ducasa G, Mallela S, Varona Santos J, Mitrofanova A, Kim J, Liu X, Sloan A, Mendez A, Banerjee S, Liu S, Szeto H, Shin M, Hoek M, Kopp J, Fontanesi F, Merscher S, Fornoni A
Human Molecular Genetics 2021;30(3-4):182-197

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Lack of APOL1 in proximal tubules of normal human kidneys and proteinuric APOL1 transgenic mouse kidneys

Dryer S, Blessing N, Wu Z, Madhavan S, Choy J, Chen M, Shin M, Hoek M, Sedor J, O’Toole J, Bruggeman L
PLOS ONE 2021;16(6):e0253197

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Recessive, gain-of-function toxicity in an APOL1 BAC transgenic mouse model mirrors human APOL1 kidney disease

McCarthy G, Blasio A, Donovan O, Schaller L, Bock-Hughes A, Magraner J, Suh J, Tattersfield C, Stillman I, Shah S, Zsengeller Z, Subramanian B, Friedman D, Pollak M
Disease Models & Mechanisms 2021;14(8)

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Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes

Scales S, Gupta N, De Mazière A, Posthuma G, Chiu C, Pierce A, Hötzel K, Tao J, Foreman O, Koukos G, Oltrabella F, Klumperman J, Lin W, Peterson A
Journal of the American Society of Nephrology 2020;31(9):2044-2064

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Profiling APOL1 Nephropathy Risk Variants in Genome-Edited Kidney Organoids with Single-Cell Transcriptomics

Liu E, Radmanesh B, Chung B, Donnan M, Yi D, Dadi A, Smith K, Himmelfarb J, Li M, Freedman B, Lin J
Kidney360 2020;1(3):203-215

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Nucleosomal dsDNA Stimulates APOL1 Expression in Human Cultured Podocytes by Activating the cGAS/IFI16-STING Signaling Pathway

Davis S, Khatua A, Popik W
Scientific Reports 2019;9(1)

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Antisense oligonucleotide treatment ameliorates IFN-γ–induced proteinuria in APOL1-transgenic mice

Aghajan M, Booten S, Althage M, Hart C, Ericsson A, Maxvall I, Ochaba J, Menschik-Lundin A, Hartleib J, Kuntz S, Gattis D, Ahlström C, Watt A, Engelhardt J, Monia B, Magnone M, Guo S
JCI Insight 2019;4(12)

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APOL1 Kidney Risk Variants Induce Cell Death via Mitochondrial Translocation and Opening of the Mitochondrial Permeability Transition Pore

Shah S, Lannon H, Dias L, Zhang J, Alper S, Pollak M, Friedman D
Journal of the American Society of Nephrology 2019;30(12):2355-2368

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APOL1 renal risk variants promote cholesterol accumulation in tissues and cultured macrophages from APOL1 transgenic mice

Long D, Ryu J, Ge M, Merscher S, Rosenberg A, Desante M, Roshanravan H, Okamoto K, Shin M, Hoek M, Fornoni A, Kopp J
PLOS ONE 2019;14(4):e0211559

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Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity

Chun J, Zhang J, Wilkins M, Subramanian B, Riella C, Magraner J, Alper S, Friedman D, Pollak M
Proceedings of the National Academy of Sciences 2019;116(9):3712-3721

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Blocking the 5′ splice site of exon 4 by a morpholino oligomer triggers APOL1 protein isoform switch

Cheatham A, Davis S, Khatua A, Popik W
Scientific Reports 2018;8(1)

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A null variant in the apolipoprotein L3 gene is associated with non-diabetic nephropathy

Skorecki K, Lee J, Langefeld C, Rosset S, Tzur S, Wasser W, Shemer R, Hawkins G, Divers J, Parekh R, Li M, Sampson M, Kretzler M, Pollak M, Shah S, Blackler D, Nichols B, Wilmot M, Alper S, Freedman B, Friedman D
Nephrology Dialysis Transplantation 2018;33(2):323-330

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APOL1 risk allele RNA contributes to renal toxicity by activating protein kinase R

Okamoto K, Rausch J, Wakashin H, Fu Y, Chung J, Dummer P, Shin M, Chandra P, Suzuki K, Shrivastav S, Rosenberg A, Hewitt S, Ray P, Noiri E, Le Grice S, Hoek M, Han Z, Winkler C, Kopp J
Communications Biology 2018;1(1)

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APOL1–Mediated Cell Injury Involves Disruption of Conserved Trafficking Processes

Kruzel-Davila E, Shemer R, Ofir A, Bavli-Kertselli I, Darlyuk-Saadon I, Oren-Giladi P, Wasser W, Magen D, Zaknoun E, Schuldiner M, Salzberg A, Kornitzer D, Marelja Z, Simons M, Skorecki K
Journal of the American Society of Nephrology 2017;28(4):1117-1130

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Intracellular APOL1 Risk Variants Cause Cytotoxicity Accompanied by Energy Depletion

Granado D, Müller D, Krausel V, Kruzel-Davila E, Schuberth C, Eschborn M, Wedlich-Söldner R, Skorecki K, Pavenstädt H, Michgehl U, Weide T
Journal of the American Society of Nephrology 2017;28(11):3227-3238

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A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease

Hayek S, Koh K, Grams M, Wei C, Ko Y, Li J, Samelko B, Lee H, Dande R, Lee H, Hahm E, Peev V, Tracy M, Tardi N, Gupta V, Altintas M, Garborcauskas G, Stojanovic N, Winkler C, Lipkowitz M, Tin A, Inker L, Levey A, Zeier M, Freedman B, Kopp J, Skorecki K, Coresh J, Quyyumi A, Sever S, Reiser J
Nature Medicine 2017;23(8):945-953

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Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice

Beckerman P, Bi-Karchin J, Park A, Qiu C, Dummer P, Soomro I, Boustany-Kari C, Pullen S, Miner J, Hu C, Rohacs T, Inoue K, Ishibe S, Saleem M, Palmer M, Cuervo A, Kopp J, Susztak K
Nature Medicine 2017;23(4):429-438

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APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8

Madhavan S, O’Toole J, Konieczkowski M, Barisoni L, Thomas D, Ganesan S, Bruggeman L, Buck M, Sedor J
JCI Insight 2017;2(14)

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APOLs with low pH dependence can kill all African trypanosomes

Fontaine F, Lecordier L, Vanwalleghem G, Uzureau P, Van Reet N, Fontaine M, Tebabi P, Vanhollebeke B, Büscher P, Pérez-Morga D, Pays E
Nature Microbiology 2017;2(11):1500-1506

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Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells

Cheng D, Weckerle A, Yu Y, Ma L, Zhu X, Murea M, Freedman B, Parks J, Shelness G
Journal of Lipid Research 2015;56(8):1583-1593

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BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins

Heneghan J, Vandorpe D, Shmukler B, Giovinazzo J, Raper J, Friedman D, Pollak M, Alper S
American Journal of Physiology-Cell Physiology 2015;309(5):C332-C347

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Exon 4-encoded sequence is a major determinant of cytotoxicity of apolipoprotein L1

Khatua A, Cheatham A, Kruzel E, Singhal P, Skorecki K, Popik W
American Journal of Physiology-Cell Physiology 2015;309(1):C22-C37

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Localization of APOL1 Protein and mRNA in the Human Kidney

Ma L, Shelness G, Snipes J, Murea M, Antinozzi P, Cheng D, Saleem M, Satchell S, Banas B, Mathieson P, Kretzler M, Hemal A, Rudel L, Petrovic S, Weckerle A, Pollak M, Ross M, Parks J, Freedman B
Journal of the American Society of Nephrology 2015;26(2):339-348

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APOL1 Null Alleles from a Rural Village in India Do Not Correlate with Glomerulosclerosis

Rastaldi M, Johnstone D, Shegokar V, Nihalani D, Rathore Y, Mallik L, Ashish , Zare V, Ikizler H, Powar R, Holzman L
PLoS ONE 2012;7(12):e51546

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APOL1 Localization in Normal Kidney and Nondiabetic Kidney Disease

Madhavan S, O'Toole J, Konieczkowski M, Ganesan S, Bruggeman L, Sedor J
Journal of the American Society of Nephrology 2011;22(11):2119-2128