H00000259-M01
antibody from Novus Biologicals
Targeting: AMBP
EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000259-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000259-M01, RRID:AB_536766
- Product name
- Mouse Monoclonal alpha 1-Microglobulin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AMBP - alpha-1-microglobulin/bikunin precursor
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Differential proteomics analysis of amniotic fluid in pregnancies of increased nuchal translucency with normal karyotype.
Cheng PJ, Wang TH, Huang SY, Kao CC, Lu JH, Hsiao CH, Shaw SW
Prenatal diagnosis 2011 Mar;31(3):274-81
Prenatal diagnosis 2011 Mar;31(3):274-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of AMBP expression in transfected 293T cell line by AMBP monoclonal antibody (M01), clone 3F1.Lane 1: AMBP transfected lysate(39 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - AMBP monoclonal antibody (M01), clone 3F1 Analysis of AMBP expression in A-549.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Detection limit for recombinant GST tagged AMBP is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of monoclonal antibody to AMBP on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha 1-Microglobulin Antibody (3F1) [H00000259-M01] - Analysis of monoclonal antibody to AMBP on formalin-fixed paraffin-embedded human liver. Antibody concentration 1 ug/ml.