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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- RQ4535 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Alpha 1 microglobulin Antibody / AMBP / Bikunin
- Antibody type
- Polyclonal
- Description
- This highly specific Alpha 1 microglobulin antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Alpha 1 microglobulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) HEp-2, 3) HeLa, 4) A549 and 5) PANC-1 lysate with Alpha 1 microglobulin antibody at 0.5ug/ml. Predicted molecular weight: 26-28 kDa (Alpha 1 microglobulin), ~39 kDa (uncleaved AMBP). These proteins may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
