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antibody from Invitrogen Antibodies
Targeting: AMBP
EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-1 Microglobulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human AMBP/Alpha 1 microglobulin.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha-1 Microglobulin in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:39 kDa. Observed band size:34 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 34 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human alpha-1-microglobulin in human liver with alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336, 1:10,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha-1 Microglobulin Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 0.5 µL with alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) and 15 µL of 50 % Protein G agarose. Primary antibody: alpha-1 Microglobulin Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.
