PAB28448

antibody from Abnova Corporation

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28448

Provider

Abnova Corporation

Product name

ATP5B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ATP5B.

Antigen sequence

TSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILN
ALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGL
VRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDE
RGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKV
VD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ATP5B polyclonal antibody (Cat # PAB28448) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS with ATP5B polyclonal antibody (Cat # PAB28448) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum with ATP5B polyclonal antibody (Cat # PAB28448) shows strong cytoplasmic positivity in glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)