PAB28383

antibody from Abnova Corporation

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28383

Provider

Abnova Corporation

Product name

ATP5B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ATP5B.

Antigen sequence

LTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEV
SALLGRIPSAVGYQPTLATDMGTMQERITTTKKGS
ITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRA
IAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVAR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ATP5B polyclonal antibody (Cat # PAB28383) at 1:100-1:250 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with ATP5B polyclonal antibody (Cat # PAB28383) at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ATP5B polyclonal antibody (Cat # PAB28383) at 1-4 ug/ml shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with ATP5B polyclonal antibody (Cat # PAB28383) shows strong cytoplasmic positivity in hepatocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)