17247-1-AP

antibody from Proteintech Group

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17247-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17247-1-AP, RRID:AB_2061878

Product name

ATPB antibody

Antibody type

Polyclonal

Description

KD/KO validated ATPB antibody (Cat. #17247-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients.

Li T, Bao Y, Xia Y, Meng H, Zhou C, Huang L, Wang X, Lai EY, Jiang P, Mao J
International journal of biological sciences 2024;20(3):937-952

---

Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation.

Wang M, Wang J, Wang L, Feng X, Qian Y, Ye C, Wang C
Phytotherapy research : PTR 2024 Feb;38(2):839-855

---

Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis.

He Y, Zhu G, Li X, Zhou M, Guan MX
iScience 2024 Feb 16;27(2):108883

---

ITFG2, an immune-modulatory protein, targets ATP 5b to maintain mitochondrial function in myocardial infarction.

Bi FF, Cao M, Pan QM, Jing ZH, Lv LF, Liu F, Tian H, Yu T, Li TY, Li XL, Liang HH, Shan HL, Zhou YH
Biochemical pharmacology 2024 Aug;226:116338

---

Tiliroside attenuates acute kidney injury by inhibiting ferroptosis through the disruption of NRF2-KEAP1 interaction.

Cai F, Li D, Zhou K, Zhang W, Yang Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Apr;126:155407

---

Olesoxime protects against cisplatin-induced acute kidney injury by attenuating mitochondrial dysfunction.

Wang P, Ouyang J, Zhou K, Hu D, Zhang S, Zhang A, Yang Y
Biomedical journal 2024 Apr 19;:100730

---

Progressive mitochondrial dysfunction in cerebellar synaptosomes of cystatin B-deficient mice.

Gorski K, Jackson CB, Nyman TA, Rezov V, Battersby BJ, Lehesjoki AE
Frontiers in molecular neuroscience 2023;16:1175851

---

WT1(+) glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury.

Hong X, Nie H, Deng J, Liang S, Chen L, Li J, Gong S, Wang G, Zuo W, Hou F, Zhang F
Theranostics 2023;13(4):1311-1324

---

F-ATP synthase inhibitory factor 1 regulates metabolic reprogramming involving its interaction with c-Myc and PGC1α.

Guo L, Gu Z
Frontiers in oncology 2023;13:1207603

---

TRPM8 promotes hepatocellular carcinoma progression by inducing SNORA55 mediated nuclear-mitochondrial communication.

Fu J, Liu G, Zhang X, Lei X, Liu Q, Qian K, Tong Q, Qin W, Li Z, Cao Z, Zhang J, Liu C, Wang Z, Liu Z, Liang XM, Yamamoto H, Xu X
Cancer gene therapy 2023 May;30(5):738-751

---

Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.

Feng X, Cheng XT, Zheng P, Li Y, Hakim J, Zhang SQ, Anderson SM, Linask K, Prestil R, Zou J, Sheng ZH, Blackstone C
Brain : a journal of neurology 2023 Jan 5;146(1):278-294

---

Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development.

Shen M, Sirois CL, Guo Y, Li M, Dong Q, Méndez-Albelo NM, Gao Y, Khullar S, Kissel L, Sandoval SO, Wolkoff NE, Huang SX, Xu Z, Bryan JE, Contractor AM, Korabelnikov T, Glass IA, Doherty D, Birth Defects Research Laboratory, Levine JE, Sousa AMM, Chang Q, Bhattacharyya A, Wang D, Werling DM, Zhao X
Neuron 2023 Dec 20;111(24):3988-4005.e11

---

Prolonged fluoride exposure induces spatial-memory deficit and hippocampal dysfunction by inhibiting small heat shock protein 22 in mice.

Xin J, Zhu B, Wang H, Zhang Y, Sun N, Cao X, Zheng L, Zhou Y, Fang J, Jing B, Pan K, Zeng Y, Zeng D, Li F, Xia Y, Xu P, Ni X
Journal of hazardous materials 2023 Aug 15;456:131595

---

NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity.

Zhao T, Zhang J, Lei H, Meng Y, Cheng H, Zhao Y, Geng G, Mu C, Chen L, Liu Q, Luo Q, Zhang C, Long Y, Su J, Wang Y, Li Z, Sun J, Chen G, Li Y, Liao X, Shang Y, Hu G, Chen Q, Zhu Y
The EMBO journal 2023 Aug 15;42(16):e113258

---

Saturated fatty acids increase LPI to reduce FUNDC1 dimerization and stability and mitochondrial function.

Chen L, Zhang Q, Meng Y, Zhao T, Mu C, Fu C, Deng C, Feng J, Du S, Liu W, Geng G, Ma K, Cheng H, Liu Q, Luo Q, Zhang J, Du Z, Cao L, Wang H, Liu Y, Lin J, Chen G, Liu L, Lam SM, Shui G, Zhu Y, Chen Q
EMBO reports 2023 Apr 5;24(4):e54731

---

Mitochondrial Targeted Antioxidant SKQ1 Ameliorates Acute Kidney Injury by Inhibiting Ferroptosis.

Song J, Sheng J, Lei J, Gan W, Yang Y
Oxidative medicine and cellular longevity 2022;2022:2223957

---

Human TRUB1 is a highly conserved pseudouridine synthase responsible for the formation of Ψ55 in mitochondrial tRNAAsn, tRNAGln, tRNAGlu and tRNAPro.

Jia Z, Meng F, Chen H, Zhu G, Li X, He Y, Zhang L, He X, Zhan H, Chen M, Ji Y, Wang M, Guan MX
Nucleic acids research 2022 Sep 9;50(16):9368-9381

---

A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease.

Shang Y, Sun X, Chen X, Wang Q, Wang EJ, Miller E, Xu R, Pieper AA, Qi X
Acta neuropathologica 2022 Nov;144(5):911-938

---

Hypoxic mesenchymal stem cell-derived extracellular vesicles ameliorate renal fibrosis after ischemia-reperfusion injure by restoring CPT1A mediated fatty acid oxidation.

Gao Z, Zhang C, Peng F, Chen Q, Zhao Y, Chen L, Wang X, Chen X
Stem cell research & therapy 2022 May 7;13(1):191

---

ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.

Zhao Y, Hu D, Wang R, Sun X, Ropelewski P, Hubler Z, Lundberg K, Wang Q, Adams DJ, Xu R, Qi X
Nature communications 2022 Mar 2;13(1):1121

---

Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases.

Fu J, Zhou M, Gritsenko MA, Nakayasu ES, Song L, Luo ZQ
eLife 2022 Jan 27;11

---

Elucidation of the Role of FAM210B in Mitochondrial Metabolism and Erythropoiesis.

Suzuki C, Fujiwara T, Shima H, Ono K, Saito K, Kato H, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Nakamura Y, Igarashi K, Harigae H
Molecular and cellular biology 2022 Dec 15;42(12):e0014322

---

TMT-Based Proteomic Explores the Influence of DHEA on the Osteogenic Differentiation of hBMSCs.

Liang X, He M, Zhu B, Zhu Y, He X, Liu D, Wei Q
Frontiers in cell and developmental biology 2021;9:726549

---

Integrated Analyses Reveal the Multi-Omics and Prognostic Characteristics of ATP5B in Breast Cancer.

Liu M, Xu Y, Zhou Y, Lang R, Shi Z, Zhao J, Meng Y, Bao L
Frontiers in genetics 2021;12:652474

---

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, Lundberg K, Adams DJ, Qi X
Nature communications 2021 Sep 6;12(1):5305

---

3D-printing magnesium-polycaprolactone loaded with melatonin inhibits the development of osteosarcoma by regulating cell-in-cell structures.

Zhang W, Zhao W, Li Q, Zhao D, Qu J, Yuan Z, Cheng Z, Zhu X, Zhuang X, Zhang Z
Journal of nanobiotechnology 2021 Sep 4;19(1):263

---

Design, synthesis, and target identification of new hypoxia-inducible factor 1 (HIF-1) inhibitors containing 1-alkyl-1H-pyrazole-3-carboxamide moiety.

Sakai M, Takahashi N, Ikeda H, Furutani Y, Higuchi S, Suzuki T, Dohmae N, Kobayashi S, Harada H, Kojima S, Matsuura T, Hattori A, Kakeya H
Bioorganic & medicinal chemistry 2021 Sep 15;46:116375

---

Leber's Hereditary Optic Neuropathy Arising From the Synergy Between ND1 3635G>A Mutation and Mitochondrial YARS2 Mutations.

Jin X, Zhang J, Yi Q, Meng F, Yu J, Ji Y, Mo JQ, Tong Y, Jiang P, Guan MX
Investigative ophthalmology & visual science 2021 Jun 1;62(7):22

---

MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.

Wang F, Chen X, Cheng H, Song L, Liu J, Caplan S, Zhu L, Wu JY
EMBO reports 2021 Jul 5;22(7):e52006

---

P2X7 receptor signaling promotes inflammation in renal parenchymal cells suffering from ischemia-reperfusion injury.

Qian Y, Qian C, Xie K, Fan Q, Yan Y, Lu R, Wang L, Zhang M, Wang Q, Mou S, Dai H, Ni Z, Pang H, Gu L
Cell death & disease 2021 Jan 27;12(1):132

---

Inhibition of the mitochondrial ATPase function by IF1 changes the spatiotemporal organization of ATP synthase.

Weissert V, Rieger B, Morris S, Arroum T, Psathaki OE, Zobel T, Perkins G, Busch KB
Biochimica et biophysica acta. Bioenergetics 2021 Jan 1;1862(1):148322

---

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Zuo X, Zhou J, Li Y, Wu K, Chen Z, Luo Z, Zhang X, Liang Y, Esteban MA, Zhou Y, Fu XD
Nature structural & molecular biology 2021 Feb;28(2):132-142

---

Mitochondrial F(1) F(O) ATP synthase determines the local proton motive force at cristae rims.

Rieger B, Arroum T, Borowski MT, Villalta J, Busch KB
EMBO reports 2021 Dec 6;22(12):e52727

---

Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase.

Reisman BJ, Guo H, Ramsey HE, Wright MT, Reinfeld BI, Ferrell PB, Sulikowski GA, Rathmell WK, Savona MR, Plate L, Rubinstein JL, Bachmann BO
Nature chemical biology 2021 Dec 2;

---

In vivo alterations of mitochondrial activity and amyloidosis in early-stage senescence-accelerated mice: a positron emission tomography study.

Yamagishi S, Iga Y, Ikegaya S, Kakiuchi T, Ohba H, Nishiyama S, Fukomoto D, Kanazawa M, Harada N, Tsukada H, Sato K, Ouchi Y
Journal of neuroinflammation 2021 Dec 10;18(1):288

---

Shen Shuai II Recipe Attenuates Apoptosis in 5/6 Renal Ablation/Infarction Rats by Inhibiting p53 and the Mitochondrial Pathway of Apoptosis.

Wang M, Yang J, Wang C
Oxidative medicine and cellular longevity 2020;2020:7083575

---

Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.

Yang Y, Liu S, Gao H, Wang P, Zhang Y, Zhang A, Jia Z, Huang S
Free radical biology & medicine 2020 May 20;152:821-837

---

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Yang L, Liu Q, Zhang X, Liu X, Zhou B, Chen J, Huang D, Li J, Li H, Chen F, Liu J, Xing Y, Chen X, Su S, Song E
Nature 2020 Jul;583(7814):133-138

---

The spatio-temporal organization of mitochondrial F(1)F(O) ATP synthase in cristae depends on its activity mode.

Salewskij K, Rieger B, Hager F, Arroum T, Duwe P, Villalta J, Colgiati S, Richter CP, Psathaki OE, Enriquez JA, Dellmann T, Busch KB
Biochimica et biophysica acta. Bioenergetics 2020 Jan 1;1861(1):148091

---

Trehalose attenuates renal ischemia-reperfusion injury by enhancing autophagy and inhibiting oxidative stress and inflammation.

Liu S, Yang Y, Gao H, Zhou N, Wang P, Zhang Y, Zhang A, Jia Z, Huang S
American journal of physiology. Renal physiology 2020 Apr 1;318(4):F994-F1005

---

SIRT5 deficiency suppresses mitochondrial ATP production and promotes AMPK activation in response to energy stress.

Zhang M, Wu J, Sun R, Tao X, Wang X, Kang Q, Wang H, Zhang L, Liu P, Zhang J, Xia Y, Zhao Y, Yang Y, Xiong Y, Guan KL, Zou Y, Ye D
PloS one 2019;14(2):e0211796

---

STING directly activates autophagy to tune the innate immune response.

Liu D, Wu H, Wang C, Li Y, Tian H, Siraj S, Sehgal SA, Wang X, Wang J, Shang Y, Jiang Z, Liu L, Chen Q
Cell death and differentiation 2019 Sep;26(9):1735-1749

---

Magnesium lithospermate B attenuates renal injury in 5/6 renal ablation/infarction rats by mitochondrial pathway of apoptosis.

Wang M, Yang L, Yang J, Zhou Y, Wang C
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109316

---

Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome.

Wu H, Wang Y, Li W, Chen H, Du L, Liu D, Wang X, Xu T, Liu L, Chen Q
Autophagy 2019 Nov;15(11):1882-1898

---

Intervention of mitochondrial activity attenuates cisplatin-induced acute kidney injury.

Yang Y, Fu Y, Wang P, Liu S, Sha Y, Zhang Y, Zhang A, Jia Z, Ding G, Huang S
International urology and nephrology 2019 Jul;51(7):1207-1218

---

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Richter U, Ng KY, Suomi F, Marttinen P, Turunen T, Jackson C, Suomalainen A, Vihinen H, Jokitalo E, Nyman TA, Isokallio MA, Stewart JB, Mancini C, Brusco A, Seneca S, Lombès A, Taylor RW, Battersby BJ
Life science alliance 2019 Feb;2(1)

---

Rotenone ameliorates chronic renal injury caused by acute ischemia/reperfusion.

Zhang W, Sha Y, Wei K, Wu C, Ding D, Yang Y, Zhu C, Zhang Y, Ding G, Zhang A, Jia Z, Huang S
Oncotarget 2018 May 11;9(36):24199-24208

---

PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver.

Xiao Y, Zhou Y, Lu Y, Zhou K, Cai W
Cell death & disease 2018 Feb 7;9(2):160

---

DJ-1 modulates mitochondrial response to oxidative stress: clues from a novel diagnosis of PARK7.

Di Nottia M, Masciullo M, Verrigni D, Petrillo S, Modoni A, Rizzo V, Di Giuda D, Rizza T, Niceta M, Torraco A, Bianchi M, Santoro M, Bentivoglio AR, Bertini E, Piemonte F, Carrozzo R, Silvestri G
Clinical genetics 2017 Jul;92(1):18-25

---

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831

---

TIM29 is a subunit of the human carrier translocase required for protein transport.

Callegari S, Richter F, Chojnacka K, Jans DC, Lorenzi I, Pacheu-Grau D, Jakobs S, Lenz C, Urlaub H, Dudek J, Chacinska A, Rehling P
FEBS letters 2016 Dec;590(23):4147-4158

---

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology 2015 Oct 26;211(2):373-89

---

Quantitative changes in Gimap3 and Gimap5 expression modify mitochondrial DNA segregation in mice.

Jokinen R, Lahtinen T, Marttinen P, Myöhänen M, Ruotsalainen P, Yeung N, Shvetsova A, Kastaniotis AJ, Hiltunen JK, Öhman T, Nyman TA, Weiler H, Battersby BJ
Genetics 2015 May;200(1):221-35

---

Cruentaren A binds F1F0 ATP synthase to modulate the Hsp90 protein folding machinery.

Hall JA, Kusuma BR, Brandt GE, Blagg BS
ACS chemical biology 2014 Apr 18;9(4):976-85

---

Relevance of the inner mitochondrial membrane enzyme F1F0-ATPase as an autoantigen in autoimmune liver disorders.

Preuss B, Berg C, Dengjel J, Stevanovic S, Klein R
Liver international : official journal of the International Association for the Study of the Liver 2012 Feb;32(2):249-57

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human heart tissue were subjected to SDS PAGE followed by western blot with 17247-1-AP(ATPB antibody) at dilution of 1:500

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ATPB (IP:17247-1-AP, 3ug; Detection:17247-1-AP 1:500) with mouse heart tissue lysate 7500ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ATPB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATPB. This antibody recognizes human,mouse,rat antigen. The ATPB antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

17247-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 17247-1-AP(ATPB antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue