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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- ARP48186_T100 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP48186_T100, RRID:AB_1107040
- Product name
- ATP5B antibody - C-terminal region (ARP48186_T100)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-ATP5B antibody detects protein at predicted molecular weight using Hepg2 Cells as a positive control. Tissue/Lysate Concentration: 1.25ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- Hepg2 Cells
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 1.25ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: IPSpecies+tissue/cell type: Mouse brain homogenateHow many ug's of tissue/cell lysate run on the gel: 500µg mouse brain homogenateIP antibody: 6µg Primary antibody dilution: 1:500 Secondary antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary antibody dilution: 1:5000
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Titration:2 ug/mlPositive Control:Human brain stem cells
