AP17895PU-N

antibody from Acris Antibodies GmbH

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP17895PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP17895PU-N, RRID:AB_10654730

Product name

anti ATP synthase subunit beta (Center)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 142~171 amino acids from the Center region of Human ATP5B.

Reactivity

Human

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

A: Western blot analysis of ATP5B Antibody (Center) Cat.-No AP17895PU-N pre-incubated without (lane 1) and with (lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab (1:60/250 dilution). B: Western blot analysis of ATP5B (arrow) using Rabbit polyclonal ATP5B Antibody (Center) Cat.-No AP17895PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow Cytometric analysis of WiDr cells (right histogram) compared to a negative control cell using ATP5B Antibody (Center) Cat.-No AP17895PU-N (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.