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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81939 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81939, RRID:AB_11162112
- Product name
- ATP5B antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5B Antibody (Center) Pab (GTX81939) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center) (GTX81939). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2) .
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center)(GTX81939), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATP5B Antibody (Center) (GTX81939) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
