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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- GTX84847 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84847, RRID:AB_10729247
- Product name
- ATP5B antibody [9E3]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B .
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ATP5B mouse monoclonal antibody (GTX84847) immunofluorescent staining of COS7 cells transiently transfected with ATP5B
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (GTX84847).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-ATP5B mouse monoclonal antibody. (GTX84847, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-ATP5B mouse monoclonal antibody. (GTX84847, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (GTX84847, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-ATP5B antibody(GTX84847),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(GTX84847),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
