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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [11]
- Flow cytometry [1]
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- Product number
- GTX84849 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84849, RRID:AB_10730698
- Product name
- ATP5B antibody [4E5]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
Submitted references Proteomic Analysis of One-carbon Metabolism-related Marker in Liver of Rat Offspring.
You YA, Lee JH, Kwon EJ, Yoo JY, Kwon WS, Pang MG, Kim YJ
Molecular & cellular proteomics : MCP 2015 Nov;14(11):2901-9
Molecular & cellular proteomics : MCP 2015 Nov;14(11):2901-9
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Supportive validation
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- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
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- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Supportive validation
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- Experimental details
- Anti-ATP5B mouse monoclonal antibody (GTX84849) immunofluorescent staining of COS7 cells transiently transfected with ATP5B
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84849, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue using anti-ATP5B mouse monoclonal antibody. (GTX84849, Dilution 1:50)
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(GTX84849),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
