HPA001520

antibody from Atlas Antibodies

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA001520

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001520, RRID:AB_1078243

Product name

Anti-ATP5B

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ATP5B, Gene description: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, Alternative Gene Names: ATPSB, Validated applications: WB, IHC, ICC, Uniprot ID: P06576, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Melatonin Reduces Mito‐Inflammation in Ischaemic Hippocampal HT22 Cells and Modulates the cGAS–STING Cytosolic DNA Sensing Pathway and FGF21 Release

Carloni S, Nasoni M, Casabianca A, Orlandi C, Capobianco L, Iaconisi G, Cerioni L, Burattini S, Benedetti S, Reiter R, Balduini W, Luchetti F
Journal of Cellular and Molecular Medicine 2024;28(24)

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Sirtuin3 ensures the metabolic plasticity of neurotransmission during glucose deprivation

Tiwari A, Hashemiaghdam A, Laramie M, Maschi D, Haddad T, Stunault M, Bergom C, Javaheri A, Klyachko V, Ashrafi G
Journal of Cell Biology 2024;223(1)

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Tiwari A, Myeong J, Hashemiaghdam A, Zhang H, Niu X, Laramie M, Stunault M, Sponagel J, Patti G, Shriver L, Klyachko V, Ashrafi G
2024

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1,4-dihydroxy quininib modulates the secretome of uveal melanoma tumour explants and a marker of oxidative phosphorylation in a metastatic xenograft model

Slater K, Bosch R, Smith K, Jahangir C, Garcia-Mulero S, Rahman A, O’Connell F, Piulats J, O’Neill V, Horgan N, Coupland S, O’Sullivan J, Gallagher W, Villanueva A, Kennedy B
Frontiers in Medicine 2023;9

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C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization

Desmurs M, Foti M, Raemy E, Vaz F, Martinou J, Bairoch A, Lane L
Molecular and Cellular Biology 2023;35(7):1139-1156

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Altered Mitochondrial Dynamic in Lymphoblasts and Fibroblasts Mutated for FANCA-A Gene: The Central Role of DRP1

Bertola N, Bruno S, Capanni C, Columbaro M, Mazzarello A, Corsolini F, Regis S, Degan P, Cappelli E, Ravera S
International Journal of Molecular Sciences 2023;24(7):6557

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ATP5B Is an Essential Factor for Hepatitis B Virus Entry

Ueda K, Suwanmanee Y
International Journal of Molecular Sciences 2022;23(17):9570

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Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma

Bertola N, Degan P, Cappelli E, Ravera S
Cells 2022;11(15):2353

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Profiling of rotavirus 3′UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication

Ren L, Ding S, Song Y, Li B, Ramanathan M, Co J, Amieva M, Khavari P, Greenberg H
Journal of Biological Chemistry 2019;294(15):5993-6006

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Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells

Bajzikova M, Kovarova J, Coelho A, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal G, Magalhaes Novais S, Olsinova M, Krobova L, An Y, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim T, Hozak P, Sardao V, Koopman W, Ricchetti M, Oliveira P, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge M, Park S, Dong L, Rohlena J, Neuzil J
Cell Metabolism 2019;29(2):399-416.e10

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Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints

Bezawork-Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, Krobova L, Vanova K, Zobalova R, Sobol M, Hozak P, Novais S, Caisova V, Abaffy P, Naraine R, Pang Y, Zaw T, Zhang P, Sindelka R, Kubista M, Zuryn S, Molloy M, Berridge M, Pacak K, Rohlena J, Park S, Neuzil J
Nature Communications 2018;9(1)

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Miro phosphorylation sites regulate Parkin recruitment and mitochondrial motility

Shlevkov E, Kramer T, Schapansky J, LaVoie M, Schwarz T
Proceedings of the National Academy of Sciences 2016;113(41)

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Resveratrol induces SIRT1- and energy–stress-independent inhibition of tumor cell regrowth after low-dose platinum treatment

Björklund M, Roos J, Gogvadze V, Shoshan M
Cancer Chemotherapy and Pharmacology 2011;68(6):1459-1467

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Surrogate antigens as targets for proteome-wide binder selection

Gustavsson E, Ek S, Steen J, Kristensson M, Älgenäs C, Uhlén M, Wingren C, Ottosson J, Hober S, Borrebaeck C
New Biotechnology 2011;28(4):302-311

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol